|
| Status |
Public on Dec 21, 2012 |
| Title |
Kb102 Testosterone |
| Sample type |
RNA |
| |
|
| Source name |
Kb102 Testosterone
|
| Organism |
Mus musculus |
| Characteristics |
cell type: sertoli cells
|
| Growth protocol |
Sertoli Cells isolated from adult mouse
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We euthanized mice by asphyxiation with CO2, harvested their testes, and isolated Sertoli cells as previously described [Chang et al., 2011; Anway et al., 2003]. We extracted RNA from purified Sertoli cells (pooled from five to seven mice) by using a Qiazol miRNeasy kit (Qiagen, Valencia, CA). We converted 1 μg of total RNA into cDNA by using a MiScript cDNA synthesis kit (Qiagen) and analyzed with quantitative real-time RT-PCR using miRNA-specific primers. We normalized miRNA expression levels to RNU19/5S and gene expression to 18S.
|
| Label |
cy5
|
| Label protocol |
Lableing was performed according to the manufacturer's directions (Agilient Inc.).
|
| |
|
| Hybridization protocol |
Hybridization, washing and scanning were performed according to the manufacturer's directions (Agilient Inc.)
|
| Scan protocol |
Hybridization, washing and scanning were performed according to the manufacturer's directions (Agilient Inc.)
|
| Data processing |
Expression values were normalized by Quantile Normalization (MATLAB Bioinformatics Toolbox, R2011a).
|
| |
|
| Submission date |
May 01, 2012 |
| Last update date |
Jun 27, 2018 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
|
| Phone |
2105629163
|
| Organization name |
UT Health Science Center at San Antonio
|
| Department |
Population Health Sciences
|
| Street address |
8403 Floyd Curl Drive, MSC 7784
|
| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
|
| Platform ID |
GPL8824 |
| Series (1) |
| GSE37679 |
Androgen-Responsive microRNAs in mouse Sertoli Cells |
|
