|
| Status |
Public on May 02, 2012 |
| Title |
RCA1 mutant, replicate 1 |
| Sample type |
SRA |
| |
|
| Source name |
mid exponential growth-phase culture in YEPD
|
| Organism |
Candida albicans |
| Characteristics |
genotype/variation: RCA1 mutant
|
| Growth protocol |
C. albicans cells were grown in YEPD liquid medium until mid exponential growth phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Yeast cells were harvested, washed, and total RNAs were extracted following standard phenol/chloroform extraction and lithium cholride precipitation. Strand specific librairies were prepared from 2 µg of total RNA following Illumina instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
117-1
|
| Data processing |
purity filtered reads were aligned to C. albicans genome using TopHat
number of read count per gene was normalized with HTseq
normalization and differential expression were analyzed with R Deseq package
Genome_build: assembly 21 s01-m02-r04
Supplementary_files_format_and_content: RAW.txt: full differential expression results
Supplementary_files_format_and_content: FC2_p0.05.txt: differential expression results, filtered on fold change > 2 and p value < 0.05
|
| |
|
| Submission date |
May 01, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Dominique Sanglard |
| E-mail(s) |
Dominique.Sanglard@chuv.ch
|
| URL |
http://www.chuv.ch/imul
|
| Organization name |
University of Lausanne and University Hospital Center
|
| Street address |
Rue Bugnon 48
|
| City |
Lausanne |
| ZIP/Postal code |
1011 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL15149 |
| Series (1) |
| GSE37682 |
Identification and functional characterization of Rca1, a transcription factor involved in both antifungal susceptibility and host response in Candida albicans |
|
| Relations |
| SRA |
SRX145373 |
| BioSample |
SAMN00990512 |
