|
| Status |
Public on Jun 01, 2012 |
| Title |
Presorted ES cells |
| Sample type |
RNA |
| |
|
| Source name |
Presorted ES cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: cultured embryonic stem (ES)
cell lineage: Presorted ES cells
|
| Treatment protocol |
Cells were differentiated in culture along the hematopoietic lineage. Cells from distinct differentiation stages were collected and RNA prepared.
|
| Growth protocol |
ES cells were grown under standard conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol was used for RNA isolation
|
| Label |
Cy3
|
| Label protocol |
Total RNA was labeled with Cy3 and RNA Ligase, as published (Thomson JM, et al, Nat Methods 1,47-53,2004).
|
| |
|
| Hybridization protocol |
RNA was hybridized to spotted microRNA array, as described in Thomson et al.
|
| Scan protocol |
Arrays were scanned on a genepix scanner.
|
| Data processing |
Raw intensity values were background subtracted, floored to 100, log transformed, and median centered by array and gene. Duplicate microRNA spots were averaged to generate the value column. Only mouse microRNAs (yes in mouse column of platform file) are presented in the value column.
|
| |
|
| Submission date |
May 02, 2012 |
| Last update date |
Jun 01, 2012 |
| Contact name |
Scott M Hammond |
| E-mail(s) |
hammond@med.unc.edu
|
| Phone |
919-843-2366
|
| Fax |
919-966-1856
|
| Organization name |
University of North Carolina
|
| Department |
Dept Cell and Developmental Biology
|
| Street address |
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL15507 |
| Series (1) |
| GSE37727 |
Primitive erythropoiesis is regulated by miR-126 via non-hematopoietic Vcam-1+ cells |
|
