GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM92666 Query DataSets for GSM92666
Status Public on Jun 27, 2006
Title cRNA self-self hybridization 1
Sample type RNA
Channel 1
Source name Ovary_epithelial_cell_line
Organism Mus musculus
Characteristics in vitro amplified RNA, replica 1
Extracted molecule total RNA
Label Cy3
Channel 2
Source name Ovary_epithelial_cell_line
Organism Mus musculus
Characteristics in vitro amplified RNA, replica1
Extracted molecule total RNA
Label Cy5
Label protocol Indirect labeling during in vitro amplification
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 ug of total RNA was amplified in 3 rounds of T7-based amplification (MessageAmpII aRNA Kit, Ambion). cRNA was indirectly labeled with Cy3/Cy5 and co-hybridised on the slide
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
Submission date Jan 17, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions

Data table
CG_Mm_3000205_1 null
CG_Mm_3000208_1 0.0274767217062437
CG_Mm_3000211_1 null
CG_Mm_3000214_1 null
CG_Mm_3000229_1 null
CG_Mm_3000232_1 null
CG_Mm_3000235_1 null
CG_Mm_3000238_1 -0.0744977784481454
CG_Mm_3000253_1 -0.158625576425318
CG_Mm_3000256_1 -0.384696387733249
CG_Mm_3000259_1 null
CG_Mm_3000262_1 null
CG_Mm_3000277_1 null
CG_Mm_3000280_1 null
CG_Mm_3000283_1 -0.0676980432021717
CG_Mm_3000286_1 null
CG_Mm_3000589_1 0.139592833872552
CG_Mm_3000592_1 -0.00087458673542784
CG_Mm_3000595_1 0.0986242365840387
CG_Mm_3000598_1 0.136235551420022

Total number of rows: 21997

Table truncated, full table size 555 Kbytes.

Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap