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Sample GSM92673 Query DataSets for GSM92673
Status Public on Jun 27, 2006
Title cRNA different samples hybridization 4
Sample type RNA
Channel 1
Source name ovary epithelium cell line
Organism Mus musculus
Characteristics in vitro amplified cRNA, replica2
Extracted molecule total RNA
Label Cy3
Label protocol Indirect labeling during in vitro amplification
Channel 2
Source name 3T3 fibroblast cell line
Organism Mus musculus
Characteristics in vitro amplified cRNA, replica 2
Extracted molecule total RNA
Label Cy5
Label protocol Indirect labeling during in vitro amplification
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 ug of total RNA from both cell lines was amplified in 3 rounds of T7-based amplification (MessageAmpII aRNA Kit, Ambion) in two parallel reactions. cRNA from 3T3 cells was indirectly labeled with Cy5, cRNA from ovary epithelium cell line (OV) was indirectrly labeled with Cy3 followed by co-hybridisation on the slide
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
Submission date Jan 17, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions

Data table
CG_Mm_3000205_1 null
CG_Mm_3000208_1 -0.644441530618229
CG_Mm_3000211_1 -0.0423092860150467
CG_Mm_3000214_1 null
CG_Mm_3000229_1 null
CG_Mm_3000232_1 null
CG_Mm_3000235_1 0.121115102331297
CG_Mm_3000238_1 null
CG_Mm_3000253_1 null
CG_Mm_3000256_1 0.0911742293021797
CG_Mm_3000259_1 null
CG_Mm_3000262_1 -0.775027979157485
CG_Mm_3000277_1 null
CG_Mm_3000280_1 -0.0806651436789828
CG_Mm_3000283_1 0.0324911349420306
CG_Mm_3000286_1 null
CG_Mm_3000589_1 0.531805427193892
CG_Mm_3000592_1 0.4087061959091
CG_Mm_3000595_1 -1.38347298209273
CG_Mm_3000598_1 -0.194945396170419

Total number of rows: 21997

Table truncated, full table size 585 Kbytes.

Supplementary data files not provided

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