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Sample GSM92677 Query DataSets for GSM92677
Status Public on Jun 27, 2006
Title GA different samples hybridization 1
Sample type RNA
Channel 1
Source name 3T3 fibroblast cell line
Organism Mus musculus
Characteristics product of GA-PCR amplification, replica1
Extracted molecule total RNA
Label Cy3
Label protocol Klenow indirect labeling
Channel 2
Source name ovary epithelium cell line
Organism Mus musculus
Characteristics product of GA-PCR amplification, replica1
Extracted molecule total RNA
Label Cy5
Label protocol Klenow indirect labeling
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 ug of total RNA from both cell lines was amplified by global poyadenilated PCR method. 3T3 DNA was labeled with Cy3, ovary DNA was labeled with Cy5 followed by co-hybridization on microarray slide.
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
Submission date Jan 17, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions

Data table
CG_Mm_3000205_1 null
CG_Mm_3000208_1 -0.118509880526674
CG_Mm_3000211_1 0.0580538869926664
CG_Mm_3000214_1 0.161866086602587
CG_Mm_3000229_1 null
CG_Mm_3000232_1 0.186034011915210
CG_Mm_3000235_1 -0.0612038599999866
CG_Mm_3000238_1 -0.242958365474136
CG_Mm_3000253_1 0.285200575352645
CG_Mm_3000256_1 -1.07314771080041
CG_Mm_3000259_1 null
CG_Mm_3000262_1 0.629056697347741
CG_Mm_3000277_1 0.524140753141184
CG_Mm_3000280_1 -0.493297333932707
CG_Mm_3000283_1 -0.0614298199902854
CG_Mm_3000286_1 null
CG_Mm_3000589_1 -0.0336659006145565
CG_Mm_3000592_1 0.230419029966651
CG_Mm_3000595_1 0.497045746000006
CG_Mm_3000598_1 -0.231699703856763

Total number of rows: 21997

Table truncated, full table size 661 Kbytes.

Supplementary data files not provided

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