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Sample GSM92703 Query DataSets for GSM92703
Status Public on Jun 27, 2006
Title GA different samples hybridization 4
Sample type RNA
Channel 1
Source name Ovary_epithelial_cell_line
Organism Mus musculus
Characteristics product of GA-PCR amplification, replica2
Extracted molecule total RNA
Label Cy3
Label protocol Klenow indirect lableling
Channel 2
Source name 3T3 fibroblast cell line
Organism Mus musculus
Characteristics product of GA-PCR amplification, replica2
Extracted molecule total RNA
Label Cy5
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10

Scan protocol
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 ng of total RNA from both cell lines was amplified by global poyadenilated PCR method in two parallel reactions. Ovary DNA was labeled with Cy3, 3T3 DNA was labeled with Cy5 followed by co-hybridization on microarray slide
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
Submission date Jan 17, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions
VALUE Ov vs 3T3

Data table
CG_Mm_3000205_1 null
CG_Mm_3000208_1 null
CG_Mm_3000211_1 -0.215863428750704
CG_Mm_3000214_1 0.157515412330923
CG_Mm_3000229_1 null
CG_Mm_3000232_1 -0.229785194575107
CG_Mm_3000235_1 -0.63684792773513
CG_Mm_3000238_1 -0.648843231795275
CG_Mm_3000253_1 -1.04725906866926
CG_Mm_3000256_1 0.557980505048939
CG_Mm_3000259_1 null
CG_Mm_3000262_1 -1.36723631951615
CG_Mm_3000277_1 -2.0048934625689
CG_Mm_3000280_1 0.168871622626961
CG_Mm_3000283_1 null
CG_Mm_3000286_1 -0.349931413868869
CG_Mm_3000589_1 0.625872068716448
CG_Mm_3000592_1 -0.489599588069049
CG_Mm_3000595_1 -1.50591711703563
CG_Mm_3000598_1 -0.350252081329936

Total number of rows: 21997

Table truncated, full table size 635 Kbytes.

Supplementary data files not provided

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