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Sample GSM92717 Query DataSets for GSM92717
Status Public on Jun 27, 2006
Title SMART different samples hybridization 1
Sample type RNA
Channel 1
Source name 3T3 fibroblast cell line
Organism Mus musculus
Characteristics Product of SMART PCR-based amplification, replica1
Extracted molecule total RNA
Label Cy3
Label protocol Klenow indirect labeling
Channel 2
Source name Ovary_epithelial_cell_line
Organism Mus musculus
Characteristics Product of SMART PCR-based amplification, replica1
Extracted molecule total RNA
Label Cy5
Label protocol Klenow indirect labeling
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 ug of total from each cell line RNA was amplified by SMART PCR-based amplificastion technique. Ovary DNA was labeled with Cy5 and 3T3 DNA was labeled with Cy3 followed by co-hybridization on microarray slide.
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
Submission date Jan 17, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions

Data table
CG_Mm_3000205_1 null
CG_Mm_3000208_1 0.182050980909044
CG_Mm_3000211_1 0.131611730319221
CG_Mm_3000214_1 0.0361228781785247
CG_Mm_3000229_1 0.142460893869024
CG_Mm_3000232_1 -0.212615044075688
CG_Mm_3000235_1 -0.0113831840850226
CG_Mm_3000238_1 -0.317861489909925
CG_Mm_3000253_1 null
CG_Mm_3000256_1 -0.165519771048922
CG_Mm_3000259_1 null
CG_Mm_3000262_1 0.197391371138970
CG_Mm_3000277_1 -0.189610098374354
CG_Mm_3000280_1 -0.131117231904891
CG_Mm_3000283_1 0.110517383752448
CG_Mm_3000286_1 0.606409325559538
CG_Mm_3000589_1 0.0945687282669724
CG_Mm_3000592_1 -0.00957340822087387
CG_Mm_3000595_1 0.420507859947125
CG_Mm_3000598_1 0.264628269648067

Total number of rows: 21997

Table truncated, full table size 761 Kbytes.

Supplementary data files not provided

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