|
| Status |
Public on May 10, 2013 |
| Title |
Non-smoker female, NSCLC, MeDIP-Seq [P8N] |
| Sample type |
SRA |
| |
|
| Source name |
Non-smoker female, NSCLC
|
| Organism |
Homo sapiens |
| Characteristics |
disease: Adenocarcinoma, NSCLC
patient: 8
tissue type: normal
Stage: IB
gender: female
age: 62 years
smoker: non-smoker
|
| Treatment protocol |
Standard protocol by phenol extraction
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Methylated CpG island-associated DNA was prepared using methylation specific antibody. This ChIPed DNA was processed for high throughput sequencing using Illumina ChIP-Seq Sample Prep Kit. For sequencing, 200bp insert sized library was selected from agarose gel. Sequencing was performed at Illumina Genome Analyzer IIx, single read sequencing.
|
| |
|
| Library strategy |
MeDIP-Seq |
| Library source |
genomic |
| Library selection |
5-methylcytidine antibody |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
62 years; female; a non-smoker
|
| Data processing |
Basecalls performed using Illumina Genome AnalyzerIIx
DNA methylation density is calculated by counting reads after binning with bin size 200bp
Genome_build: hg19
Supplementary_files_format_and_content: text
|
| |
|
| Submission date |
May 04, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Sanghyuk Lee |
| E-mail(s) |
sanghyuk@ewha.ac.kr
|
| Phone |
+82-42-879-8511
|
| Fax |
+82-42-879-8519
|
| URL |
http://www.kobic.re.kr
|
| Organization name |
KRIBB
|
| Department |
KOBIC
|
| Street address |
125 Gwahak-ro
|
| City |
Daejeon |
| ZIP/Postal code |
305-806 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL10999 |
| Series (2) |
| GSE37764 |
A high dimensional deep sequencing study of non-small cell lung adenocarcinoma in never-smoker Korean females [Seq] |
| GSE37765 |
A high dimensional deep sequencing study of non-small cell lung adenocarcinoma in never-smoker Korean females |
|
| Relations |
| SRA |
SRX145910 |
| BioSample |
SAMN00990863 |
