Product of SMART PCR-based amplification, replica1
Extracted molecule
total RNA
Label
Cy5
Label protocol
Klenow indirect labelingSee Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Hybridization protocol
Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description
RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 ug of total from each cell line RNA was amplified by SMART PCR-based amplificastion technique. Ovary DNA was labeled with Cy3 and 3T3 DNA was labeled with Cy5 followed by co-hybridization on microarray slide.
Data processing
Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
