|
| Status |
Public on Jun 27, 2006 |
| Title |
SMART replicate hybridization 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Ovary_epithelial_cell_line
|
| Organism |
Mus musculus |
| Characteristics |
Product of SMART PCR-based amplification, replica1
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| Label protocol |
Klenow indirect labeling
|
| |
|
| Channel 2 |
| Source name |
Ovary_epithelial_cell_line
|
| Organism |
Mus musculus |
| Characteristics |
Product of SMART PCR-based amplification, replica2
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| Label protocol |
Klenow indirect labeling
|
| |
|
| |
| Hybridization protocol |
See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
|
| Scan protocol |
Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
|
| Description |
RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 ng of total RNA was amplified by SMART PCR-based amplification technique in two parallel reactions. Replica1 was labeled with Cy3 and replica2 was labeled with Cy5 followed by co-hybridization on microarray slide.
|
| Data processing |
Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
|
| |
|
| Submission date |
Jan 17, 2006 |
| Last update date |
Jun 27, 2006 |
| Contact name |
Tatiana Subkhankulova |
| E-mail(s) |
subkhankul@hotmail.com
|
| Phone |
02075941825
|
| Organization name |
Queen Mary University London
|
| Department |
Neuroscience
|
| Lab |
Silvia Marino
|
| Street address |
4 Newark Street
|
| City |
London |
| ZIP/Postal code |
E1 2AD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL2584 |
| Series (1) |
| GSE5136 |
Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level |
|
