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Sample GSM92749 Query DataSets for GSM92749
Status Public on Jun 27, 2006
Title SMART replicate hybridization 2
Sample type RNA
Channel 1
Source name Ovary_epithelial_cell_line
Organism Mus musculus
Characteristics Product of SMART PCR-based amplification, replica2
Extracted molecule total RNA
Label Cy3
Label protocol Klenow indirect labeling
Channel 2
Source name Ovary_epithelial_cell_line
Organism Mus musculus
Characteristics Product of SMART PCR-based amplification, replica1
Extracted molecule total RNA
Label Cy5
Label protocol Klenow indirect labeling
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software
Description RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156. 10 ug of total RNA was amplified by SMART PCR-based amplification technique in two parallel reactions. Replica1 was labeled with Cy5 and replica2 was labeled with Cy3 followed by co-hybridization on microarray slide.
Data processing Limma packadge: log-ratio were lowess normalized, filtered to exclude spots flagged as bad and backgroud corrected.
Submission date Jan 17, 2006
Last update date Jun 27, 2006
Contact name Tatiana Subkhankulova
Phone 02075941825
Organization name Queen Mary University London
Department Neuroscience
Lab Silvia Marino
Street address 4 Newark Street
City London
ZIP/Postal code E1 2AD
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE5136 Comparison of linear and exponential amplification techniques for expression profiling at the single-cell level

Data table header descriptions

Data table
CG_Mm_3000205_1 null
CG_Mm_3000208_1 -0.107632854985996
CG_Mm_3000211_1 0.103240507490201
CG_Mm_3000214_1 0.177710488843212
CG_Mm_3000229_1 null
CG_Mm_3000232_1 0.0934705140759035
CG_Mm_3000235_1 0.263595095153978
CG_Mm_3000238_1 -0.368759771647460
CG_Mm_3000253_1 null
CG_Mm_3000256_1 -0.178902974710258
CG_Mm_3000259_1 null
CG_Mm_3000262_1 -0.135718152438983
CG_Mm_3000277_1 -0.0125310289654699
CG_Mm_3000280_1 null
CG_Mm_3000283_1 null
CG_Mm_3000286_1 null
CG_Mm_3000589_1 0.224395221069827
CG_Mm_3000592_1 0.0593912257224074
CG_Mm_3000595_1 0.0838597127411967
CG_Mm_3000598_1 0.274373443520518

Total number of rows: 21997

Table truncated, full table size 680 Kbytes.

Supplementary data files not provided

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