|
Status |
Public on Jul 01, 2012 |
Title |
Sarcoidosis 14 |
Sample type |
RNA |
|
|
Source name |
EA_Complicated sarcoidosis_PBMCs
|
Organism |
Homo sapiens |
Characteristics |
ethnicity: European descent American (EA) cell type: peripheral blood mononuclear cells diagnosis: Complicated sarcoidosis
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
|
|
Hybridization protocol |
Samples were hybridized with Affymetrix GeneChip Human Exon 1.0 ST Arrays per manufacturer’s instructions.
|
Scan protocol |
Array scanning was performed according to the manufacturer's instruction by the University of Chicago Functional Genomics Facility.
|
Description |
Gene expression data from PBMC total RNA from healthy controls. Sarcoidosis_14
|
Data processing |
The transcript expression data were summarized by RMA using the Affymetrix Power Tools (v.1.12.0) and were later corrected for batch effect using the R COMBAT script.
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|
|
Submission date |
May 10, 2012 |
Last update date |
Jul 01, 2012 |
Contact name |
Tong Zhou |
Organization name |
University of Nevada School of Medicine
|
Street address |
1664 N Virginia St
|
City |
Reno |
State/province |
NV |
ZIP/Postal code |
89557 |
Country |
USA |
|
|
Platform ID |
GPL5175 |
Series (1) |
GSE37912 |
Peripheral blood gene expression as a novel genomic biomarker in complicated sarcoidosis |
|