|
| Status |
Public on Jul 01, 2012 |
| Title |
Sarcoidosis 33 |
| Sample type |
RNA |
| |
|
| Source name |
EA_Uncomplicated sarcoidosis_PBMCs
|
| Organism |
Homo sapiens |
| Characteristics |
ethnicity: European descent American (EA)
cell type: peripheral blood mononuclear cells
diagnosis: Uncomplicated sarcoidosis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Samples were hybridized with Affymetrix GeneChip Human Exon 1.0 ST Arrays per manufacturer’s instructions.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction by the University of Chicago Functional Genomics Facility.
|
| Description |
Gene expression data from PBMC total RNA from healthy controls.
Sarcoidosis_33
|
| Data processing |
The transcript expression data were summarized by RMA using the Affymetrix Power Tools (v.1.12.0) and were later corrected for batch effect using the R COMBAT script.
|
| |
|
| Submission date |
May 10, 2012 |
| Last update date |
Jul 01, 2012 |
| Contact name |
Tong Zhou |
| Organization name |
University of Nevada School of Medicine
|
| Street address |
1664 N Virginia St
|
| City |
Reno |
| State/province |
NV |
| ZIP/Postal code |
89557 |
| Country |
USA |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE37912 |
Peripheral blood gene expression as a novel genomic biomarker in complicated sarcoidosis |
|
