NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM930824 Query DataSets for GSM930824
Status Public on Mar 23, 2013
Title KYSE140 CD90-
Sample type RNA
 
Source name CD90- ESCC
Organism Homo sapiens
Characteristics cell line: KYSE-140
cell type: esophageal squamous carcinoma cell (ESCC)
cell population: CD90-
Growth protocol CD90+ and CD90- cells were sorted from KYSE-140 ESCC cells using the flow cytometry cell sorting technique.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached to the Series).
 
Hybridization protocol Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached to the Series).
Scan protocol Service by The University of Hong Kong (HKU) Genome Research Centre (protocol attached to the Series).
Description Gene expression.
Data processing The data were analyzed with MAS5 using Affymetrix default analysis settings and global scaling as the normalization method.
 
Submission date May 11, 2012
Last update date Mar 23, 2013
Contact name Stephanie Ma
E-mail(s) stefma@hku.hk
Organization name The University of Hong Kong
Department School of Biomedical Sciences
Street address Rm 47, 1/F, Lab Block, Faculty of Medicine Bldg, 21 Sassoon Rd, Pok Fu Lam
City Hong Kong
ZIP/Postal code NA
Country Hong Kong
 
Platform ID GPL570
Series (1)
GSE37949 Expression data comparing KYSE-140 ESCC cell line CD90+ and CD90- cells

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 950.746 P 0.000509415
AFFX-BioB-M_at 1175.91 P 4.42873e-05
AFFX-BioB-3_at 863.784 P 5.16732e-05
AFFX-BioC-5_at 2234.19 P 5.16732e-05
AFFX-BioC-3_at 3418.95 P 4.42873e-05
AFFX-BioDn-5_at 6833.84 P 4.42873e-05
AFFX-BioDn-3_at 12135.2 P 6.02111e-05
AFFX-CreX-5_at 29230.5 P 5.16732e-05
AFFX-CreX-3_at 29467.2 P 4.42873e-05
AFFX-DapX-5_at 125.82 P 0.000296381
AFFX-DapX-M_at 783.047 P 0.000753643
AFFX-DapX-3_at 2731.77 P 4.42873e-05
AFFX-LysX-5_at 192.517 P 5.16732e-05
AFFX-LysX-M_at 251.268 P 0.00618711
AFFX-LysX-3_at 457.226 P 4.42873e-05
AFFX-PheX-5_at 103.606 P 0.000195116
AFFX-PheX-M_at 163.985 P 0.000509415
AFFX-PheX-3_at 234.671 P 4.42873e-05
AFFX-ThrX-5_at 46.2298 A 0.131361
AFFX-ThrX-M_at 155.698 P 0.00110197

Total number of rows: 54675

Table truncated, full table size 1640 Kbytes.




Supplementary file Size Download File type/resource
GSM930824_140-CD90-_110318.CEL.gz 4.7 Mb (ftp)(http) CEL
GSM930824_140-CD90-_110318.MAS5.CHP.gz 490.3 Kb (ftp)(http) CHP
Processed data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap