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Sample GSM932000 Query DataSets for GSM932000
Status Public on Jul 03, 2012
Title BMD DNA 282
Sample type genomic
 
Source name whole blood, case
Organism Canis lupus familiaris
Characteristics breed: Bernese Mountain Dog (BMD)
phenotype: histiocytic sarcoma (HS)
origin: North America
tissue: whole blood
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was extracted from whole blood using either Proteinase K and phenol/chloroform with standard protocols (NIH and University of Utrecht) or the Nucleon BACC Genomic DNA extraction kit (University of Rennes).
Label C-Bio and A-DNP
Label protocol 200ng of genomic DNA was amplified in an overnight reaction at 37oC. The amplified DNA was enzymatically fragmented, precipitated with isopropanol, and resuspended in hybridization buffer.
 
Hybridization protocol Resuspended DNA was loaded onto BeadChips arrays and hybridized overnight at 48oC. Single-base extension and allele-specific staining were performed by hand.
Scan protocol After allele-specific staining, BeadChip arrays were coated with XC4/ethanol, dried for 1 hour and scanned on a BeadArray Reader (Illumina).
Description 282
Data processing Image data was analyzed using GenomeStudio with GT module (Illumina).
 
Submission date May 16, 2012
Last update date Jul 03, 2012
Contact name Heidi G Parker
E-mail(s) hgparker@mail.nih.gov
Organization name NHGRI
Department CGB
Lab CGS
Street address 50 South Dr. Rm 5347
City Bethesda
State/province MD
ZIP/Postal code 20874
Country USA
 
Platform ID GPL15578
Series (1)
GSE38011 The MTAP-CDKN2A Locus Confers Susceptibility to a Naturally Occurring Canine Cancer

Data table header descriptions
ID_REF
VALUE Genotype: AA, AB, BB, NC=no call
GC_SCORE
Theta
R
B_Allele_Freq
Log_R_Ratio

Data table
ID_REF VALUE GC_SCORE Theta R B_Allele_Freq Log_R_Ratio
11703 BB 0.9737642 0.9724522 0.5441412 0.9848258 -0.07111341
5137 BB 0.9613768 0.9881734 1.052532 0.9995489 -0.03125574
18822 AB 0.8390679 0.6009485 0.8804877 0.4557263 0.3229339
10277 AB 0.9219033 0.6109724 1.28675 0.507062 0.04271737
11788 BB 0.848551 0.9396623 0.4978112 0.9346456 -0.2917682
14336 AA 0.9792573 0.006145233 1.348079 0.002303478 0.08287441
7254 AA 0.8690977 0.05538559 0.2567515 0.01902541 -0.5092547
9351 BB 0.4183931 0.9942807 1.169478 1 -0.1678653
8523 AA 0.9256464 0.02436109 0.8132823 0.005729613 -0.04751478
5138 BB 0.9469349 0.9904196 0.982238 0.998417 0.005649655
5140 AA 0.9542229 0.01183961 1.092188 0.005843347 0.02939432
20166 AA 0.8710682 0.1015749 0.4752014 0.04168455 -0.293474
5141 AA 0.8660119 0.003148415 1.076742 0.002653789 0.05337942
5142 AA 0.8143145 0.003738184 0.723097 0.000699053 -0.07993431
7716 BB 0.8319238 0.9540416 1.033501 0.9499205 0.05786649
5143 BB 0.8746183 0.9940309 1.249471 1 0.04020259
5144 BB 0.8779246 0.9800539 0.7632369 0.9994485 -0.03729365
5145 AA 0.9658952 0.00208026 0.8676625 0 -0.1121027
5146 BB 0.6835921 0.9782172 1.266909 0.9963048 0.1010882
5147 AA 0.9369485 0.004175304 1.061242 6.37E-06 0.02176389

Total number of rows: 22362

Table truncated, full table size 1249 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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