|
Status |
Public on Aug 20, 2012 |
Title |
Normal_skin_rep3 |
Sample type |
RNA |
|
|
Source name |
skin tissue, tamoxifen tretaed
|
Organism |
Mus musculus |
Characteristics |
genetic background: FVB/N genotype/variation: Normal
|
Treatment protocol |
Tamoxifen treatment was topicallyadministered in the shaved backskin of animals (2x2 cm) at 20 mg/per day dissolved in DMSO/acetone for 5 consecutive days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Extraction of total RNA was performed with RNeasy Qiagen Kit according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Data processing |
The data were analyzed with RMA using RMAExpress.
|
|
|
Submission date |
May 18, 2012 |
Last update date |
Aug 23, 2012 |
Contact name |
Ramon Garcia-Escudero |
E-mail(s) |
ramon.garcia@ciemat.es, rgescudero@gmail.com
|
Organization name |
CIEMAT
|
Department |
Biomedical Innovation
|
Lab |
Molecular and Traslational Oncology
|
Street address |
Avda Complutense 40, Ed 70A
|
City |
Madrid |
State/province |
Madrid |
ZIP/Postal code |
28040 |
Country |
Spain |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE38048 |
E2F1 loss induces spontaneous tumour development in Rb-deficient epidermis |
|