|
| Status |
Public on Aug 20, 2012 |
| Title |
Rb_E2F1_skin_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
skin tissue, tamoxifen tretaed
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: FVB/N
genotype/variation: Rb_E2F1
|
| Treatment protocol |
Tamoxifen treatment was topicallyadministered in the shaved backskin of animals (2x2 cm) at 20 mg/per day dissolved in DMSO/acetone for 5 consecutive days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed with RNeasy Qiagen Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with RMA using RMAExpress.
|
| |
|
| Submission date |
May 18, 2012 |
| Last update date |
Aug 23, 2012 |
| Contact name |
Ramon Garcia-Escudero |
| E-mail(s) |
ramon.garcia@ciemat.es, rgescudero@gmail.com
|
| Organization name |
CIEMAT
|
| Department |
Biomedical Innovation
|
| Lab |
Molecular and Traslational Oncology
|
| Street address |
Avda Complutense 40, Ed 70A
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE38048 |
E2F1 loss induces spontaneous tumour development in Rb-deficient epidermis |
|
