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Sample GSM935069 Query DataSets for GSM935069
Status Public on Nov 18, 2012
Title 970
Sample type RNA
 
Source name aorta, NZW/LacJ
Organism Mus musculus
Characteristics strain: NZW/LacJ
genotype: wild-type
tissue: aorta
gender: male
age: 16 weeks
Treatment protocol None
Growth protocol 16-week-old male mice were maintained on a 12 hr light dark cycle and were fed a chow diet.
Extracted molecule total RNA
Extraction protocol Qiazol with homogenization followed by RNeasy spin columns with optional DNase per the manufacturer's protocol.
Label Biotin
Label protocol All target labeling reagents were purchased from Affymetrix (Santa Clara, CA). Double-stranded cDNAs were synthesized from 1ug total RNA through reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter and double strand conversion using the cDNA Synthesis System. Biotin-labeled cRNA was generated from the cDNA and used to probe Affymetrix Mouse Genome HT_MG-430A arrays.
 
Hybridization protocol The HT_MG-430A Array plate consists of 96 single MG-430A arrays arranged into standard SBS 96 well plate format. All cDNA and cRNA target preparation steps were processed on a Caliper GeneChip Array Station from Affymetrix. Array hybridization, washing and scanning were performed according to the manufacturer's recommendations.
Scan protocol Scanned images were subjected to visual inspection, and a chip quality report was generated by the Affymetrix's GeneChip Operating System (GCOS) and Expression Console (Affymetrix).
Description NZW_13
Data processing The image data was processed using the Affymetrix GCOS algorithm utilizing quantile normalization or the Robust Multiarray method (RMA) to determine the specific hybridizing signal for each gene.
 
Submission date May 22, 2012
Last update date Nov 18, 2012
Contact name Calvin Pan
Organization name UCLA
Street address 675 Charles Young Dr South
City Los Angeles
ZIP/Postal code 90095
Country USA
 
Platform ID GPL8759
Series (1)
GSE38120 Aorta profiling HMDP

Data table header descriptions
ID_REF
VALUE RMA-normalized expression value (log2 scale)

Data table
ID_REF VALUE
1415670_at 7.82863601843816
1415671_at 8.23254120285655
1415672_at 6.84566579090825
1415673_at 7.10123886702028
1415674_a_at 7.52167589069272
1415675_at 6.79361379906835
1415676_a_at 9.14248611762877
1415677_at 7.71876930327535
1415678_at 8.8802198390698
1415679_at 9.38060232531858
1415680_at 7.05552399891691
1415681_at 7.8665873520308
1415682_at 6.534028340379
1415683_at 9.84377090233652
1415684_at 5.70354188634048
1415685_at 7.24622370884191
1415686_at 8.28808961374672
1415687_a_at 11.2185905379163
1415688_at 9.02461164039899
1415689_s_at 7.44428499754665

Total number of rows: 22416

Table truncated, full table size 624 Kbytes.




Supplementary file Size Download File type/resource
GSM935069_5500144076463051910911_D07.cel.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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