|
Status |
Public on Nov 18, 2012 |
Title |
970 |
Sample type |
RNA |
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Source name |
aorta, NZW/LacJ
|
Organism |
Mus musculus |
Characteristics |
strain: NZW/LacJ genotype: wild-type tissue: aorta gender: male age: 16 weeks
|
Treatment protocol |
None
|
Growth protocol |
16-week-old male mice were maintained on a 12 hr light dark cycle and were fed a chow diet.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiazol with homogenization followed by RNeasy spin columns with optional DNase per the manufacturer's protocol.
|
Label |
Biotin
|
Label protocol |
All target labeling reagents were purchased from Affymetrix (Santa Clara, CA). Double-stranded cDNAs were synthesized from 1ug total RNA through reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter and double strand conversion using the cDNA Synthesis System. Biotin-labeled cRNA was generated from the cDNA and used to probe Affymetrix Mouse Genome HT_MG-430A arrays.
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|
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Hybridization protocol |
The HT_MG-430A Array plate consists of 96 single MG-430A arrays arranged into standard SBS 96 well plate format. All cDNA and cRNA target preparation steps were processed on a Caliper GeneChip Array Station from Affymetrix. Array hybridization, washing and scanning were performed according to the manufacturer's recommendations.
|
Scan protocol |
Scanned images were subjected to visual inspection, and a chip quality report was generated by the Affymetrix's GeneChip Operating System (GCOS) and Expression Console (Affymetrix).
|
Description |
NZW_13
|
Data processing |
The image data was processed using the Affymetrix GCOS algorithm utilizing quantile normalization or the Robust Multiarray method (RMA) to determine the specific hybridizing signal for each gene.
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|
|
Submission date |
May 22, 2012 |
Last update date |
Nov 18, 2012 |
Contact name |
Calvin Pan |
Organization name |
UCLA
|
Street address |
675 Charles Young Dr South
|
City |
Los Angeles |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL8759 |
Series (1) |
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