|
| Status |
Public on Nov 18, 2012 |
| Title |
972 |
| Sample type |
RNA |
| |
|
| Source name |
aorta, SM/J
|
| Organism |
Mus musculus |
| Characteristics |
strain: SM/J
genotype: wild-type
tissue: aorta
gender: male
age: 16 weeks
|
| Treatment protocol |
None
|
| Growth protocol |
16-week-old male mice were maintained on a 12 hr light dark cycle and were fed a chow diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiazol with homogenization followed by RNeasy spin columns with optional DNase per the manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
All target labeling reagents were purchased from Affymetrix (Santa Clara, CA). Double-stranded cDNAs were synthesized from 1ug total RNA through reverse transcription with an oligo-dT primer containing the T7 RNA polymerase promoter and double strand conversion using the cDNA Synthesis System. Biotin-labeled cRNA was generated from the cDNA and used to probe Affymetrix Mouse Genome HT_MG-430A arrays.
|
| |
|
| Hybridization protocol |
The HT_MG-430A Array plate consists of 96 single MG-430A arrays arranged into standard SBS 96 well plate format. All cDNA and cRNA target preparation steps were processed on a Caliper GeneChip Array Station from Affymetrix. Array hybridization, washing and scanning were performed according to the manufacturer's recommendations.
|
| Scan protocol |
Scanned images were subjected to visual inspection, and a chip quality report was generated by the Affymetrix's GeneChip Operating System (GCOS) and Expression Console (Affymetrix).
|
| Description |
SM_17
|
| Data processing |
The image data was processed using the Affymetrix GCOS algorithm utilizing quantile normalization or the Robust Multiarray method (RMA) to determine the specific hybridizing signal for each gene.
|
| |
|
| Submission date |
May 22, 2012 |
| Last update date |
Nov 18, 2012 |
| Contact name |
Calvin Pan |
| Organization name |
UCLA
|
| Street address |
675 Charles Young Dr South
|
| City |
Los Angeles |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL8759 |
| Series (1) |
|
