|
| Status |
Public on Jul 12, 2012 |
| Title |
atfs-1(tm4525)-treated, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
atfs-1(tm4525) worms on spg-7l(RNAi)
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: whole organism
genotype: atfs-1(tm4525)
age: L4
knockdown: spg-7 RNAi
genetic background: N2
|
| Treatment protocol |
Worms were fed either control (RNAi) and spg-7 (RNAi) and harvested at L4 stage
|
| Growth protocol |
Wild-type and atfs-1(tm4525) worms were synchronized by bleaching and raised in liquid culture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNA STAT reagent (Tel-Test Inc) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip C.elegans Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Addymetrix 3000 7G Scanner.
|
| Description |
Gene expression data from atfs-1(tm4525) on spg-7(RNAi)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 23, 2012 |
| Last update date |
Jul 12, 2012 |
| Contact name |
Jeffrey Zhao |
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Genomics Core
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE38196 |
ATFS-1 mediates a protective transcription program during mitochondrial stress |
|
