|
| Status |
Public on Jun 12, 2014 |
| Title |
PfBBc6R8-contact-3 |
| Sample type |
RNA |
| |
|
| Source name |
PfBBc6R8, at the time of contact with fungus
|
| Organism |
Pseudomonas fluorescens BBc6R8 |
| Characteristics |
strain: BBc6R8
treatment: Laccaria bicolor fungus
sampling time: 16 days after inoculation
|
| Biomaterial provider |
Institut National de la Recherche Agronomique (INRA Nancy, France).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen, Stockholm, Sweden) as recommended by the manufacturer. Total RNA preparations were amplified using the SMART PCR cDNA Synthesis Kit (Clontech) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimbleGen Company.
|
| |
|
| Hybridization protocol |
Hybridization by NimbleGen Company.
|
| Scan protocol |
Scanning by NimbleGen Company.
|
| Description |
PfBBc6R8-at the time of contact (16 days after inoculation)-co-culture with fungus Laccaria bicolor.
|
| Data processing |
Expression values reflect across-array quantile normalization and gene calls generated using the Robust Multichip Average (RMA). For information on RMA, see Biostatistics 2003 Apr; 4(2): 249-264.
|
| |
|
| Submission date |
May 25, 2012 |
| Last update date |
Jun 12, 2014 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
annegret.kohler@inrae.fr
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
| |
|
| Platform ID |
GPL15608 |
| Series (1) |
| GSE38243 |
Mechanisms of interaction between ectomycorrhizal fungi and the mycorrhiza helper bacterium Pseudomonas fluorescens BBc6R8 |
|
