|
| Status |
Public on Jun 01, 2013 |
| Title |
48 hpf trunk region of zebrafish embryos: WT vs MO-grnA injected Replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
MO-grnA_48 hpf
|
| Organism |
Danio rerio |
| Characteristics |
injected with: morpholino of zf-grna
age: 48 hpf
tissue: trunk region of morphant
|
| Treatment protocol |
An injection volume from 2-3 nl of MOs was injected into 1-cell-stage embryos.
|
| Growth protocol |
The zebrafish were maintained at our own facility in a controlled environment with a 14/10-h light-dark cycle at 28 °C. The fertilized eggs were collected at the 1-cell stage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Agilent's Quick Amp Labeling Kit denerates fluorescent cRNA with a sample input RNA 1 μg of total RNA for two-color processing.The method uses T7 RNA polymerase,which simultaneously amplifies target material and incorporates cyanine 3- or cyanine 5-labeled CTP.
|
| |
|
| Channel 2 |
| Source name |
WT_48 hpf
|
| Organism |
Danio rerio |
| Characteristics |
genotype/variation: wild type (untreated)
age: 48 hpf
tissue: trunk region of zebrafish embryos
|
| Treatment protocol |
An injection volume from 2-3 nl of MOs was injected into 1-cell-stage embryos.
|
| Growth protocol |
The zebrafish were maintained at our own facility in a controlled environment with a 14/10-h light-dark cycle at 28 °C. The fertilized eggs were collected at the 1-cell stage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Agilent's Quick Amp Labeling Kit denerates fluorescent cRNA with a sample input RNA 1 μg of total RNA for two-color processing.The method uses T7 RNA polymerase,which simultaneously amplifies target material and incorporates cyanine 3- or cyanine 5-labeled CTP.
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer (Agilent Gene Expression Hybridization Kit) were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially.
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
Images were quantified using Agilent Feature Extraction (FE) Software (version10.5.1.1).
|
| Description |
Biological replicate 1 of 3
48 hpf MO-grnA Rep 1
|
| Data processing |
Agilent Feature Extraction Software (v 10.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jun 04, 2012 |
| Last update date |
Jun 01, 2013 |
| Contact name |
gen hwa lin |
| E-mail(s) |
genhwa@gate.sinica.edu.tw
|
| Phone |
886-2-27899534
|
| Organization name |
Academia Sinica
|
| Department |
Institute of Cellular and Organismic Biology
|
| Lab |
Lab of Marine Molecular Biology and Biotechnology
|
| Street address |
128,section2,academia road
|
| City |
Taipei |
| State/province |
Nankang |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6457 |
| Series (1) |
| GSE38441 |
WT vs MO-grnA injected: 16, 24, 48, 72 hpf trunk region of zebrafish embryos |
|
