|
| Status |
Public on Dec 01, 2017 |
| Title |
Neonatal male HHR heart 2 |
| Sample type |
RNA |
| |
|
| Source name |
Neonatal male HHR heart
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: Male
tissue: heart
strain: HHR
age: 2 days old
heart size: Hypetrophic
|
| Treatment protocol |
Tissue was transferred either into microtubes and immediately frozen with liquid nitrogen. It was then preserved at -80°C before RNA extraction.
|
| Growth protocol |
The whole heart from neonatal animals was obtained immediately after euthanasia.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy kit (Qiagen) was used for extraction of total RNA and microRNA according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA was prepared for hybridisation according to the standard Affymetrix protocol (GeneChip® Whole Transcript (WT) Sense Target Labeling Assay Manual, version 4, Affymetrix).
|
| |
|
| Hybridization protocol |
After fragmentation, ssDNA was hybridized for 17 hr at 45°C to GeneChip Human Gene 1.0 ST Arrays. Chips were washed and stained in the Affymetrix GeneChip® Fluidics Stations 450. For all these steps, the GeneChip Hybridization, Wash, and Stain Kit (Affymetrix) was used according to manufacturer’s recommendations (GeneChip® Whole Transcript (WT) Sense Target Labelling Assay Manual, version 4, Affymetrix).
|
| Scan protocol |
The chips were scanned using the GeneChip® Scanner 3000 7G 4C (Affymetrix) according to manufacturer’s recommendations (GeneChip® Whole Transcript (WT) Sense Target Labelling Assay Manual, version 4, Affymetrix).
|
| Description |
Gene expression from neonatal male HHR heart
|
| Data processing |
The samples were individually normalized using robust-multi-array analysis (RMA) in the R application (version 2.11.1). Differentially expressed genes were identified using the moderated two-sample t-test in the Limma package.
|
| |
|
| Submission date |
Jun 08, 2012 |
| Last update date |
Dec 01, 2017 |
| Contact name |
Francine Marques |
| E-mail(s) |
Francine.marques@bakeridi.edu.au
|
| Phone |
61385321916
|
| Organization name |
Baker IDI Heart and Diabetes
|
| Street address |
Level 3, 75 Commercial Rd
|
| City |
Melbourne |
| State/province |
VIC |
| ZIP/Postal code |
3004 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE38607 |
Transcriptome-wide gene expression of the heart of neonatal rats with polygenic cardiac hypetrophy |
|
