|
| Status |
Public on Oct 04, 2006 |
| Title |
control for Cu shock treatment rep2 |
| Sample type |
RNA |
| |
|
| Source name |
control for 10mM Cu shock treatment
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
Pseudomonas aeruginosa PAO1
|
| Treatment protocol |
early log phase (OD600=0.2) treated with HCl to bring pH to same levels as with 10 mM Cu for 45 min
|
| Growth protocol |
grown in LB with 100 mM MOPS at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
2 mL of culture was added to 4 mL RNAlater (Ambion). Total RNA was extracted using an RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Approximately 36 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Cu shock control treatment
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Jan 31, 2006 |
| Last update date |
Oct 04, 2006 |
| Contact name |
Gail M Teitzel |
| E-mail(s) |
gail_teitzel@hotmail.com
|
| Phone |
773 834 1906
|
| Organization name |
University of Chicago
|
| Department |
MGCB
|
| Lab |
EBC 409
|
| Street address |
1103 E. 57th St
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE4152 |
Transcriptional profiling of copper-stressed Pseudomonas aeruginosa. |
|
