|
Status |
Public on Jun 14, 2012 |
Title |
Control rep2 |
Sample type |
RNA |
|
|
Source name |
ESC untreated control
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 cell line: ESCs stably stably expressing a chimeric receptor (GRgp-Y118F) agent: control
|
Treatment protocol |
GCSF (homemade), LIF (homemade), PD0325901 (Stemgent 1 µM)
|
Growth protocol |
GMEM medium (Sigma) supplemented with 10% fetal bovine serum (FBS) (Hyclone), 2 mM L-glutamine (Gibco), 1 mM sodium pyruvate (Gibco), 1% nonessential amino acids (Gibco), 0.1 mM β-mercaptoethanol (Sigma)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by using TRIzol (Invitrogen)
|
Label |
biotin
|
Label protocol |
Performed by UCLA DNA Microarray core facility
|
|
|
Hybridization protocol |
Performed by UCLA DNA Microarray core facility
|
Scan protocol |
Performed by UCLA DNA Microarray core facility
|
Description |
Mouse embryonic stem cell, B6
|
Data processing |
The data analysis was performed by using Microarray Я US program provided by USC Norris Medical Library
|
|
|
Submission date |
Jun 14, 2012 |
Last update date |
Jun 14, 2012 |
Contact name |
Qilong Ying |
Organization name |
USC
|
Lab |
Qilong Ying
|
Street address |
1425 san pablo st BCC509b
|
City |
los angeles |
State/province |
CA |
ZIP/Postal code |
90033 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE38719 |
Identify the downstream targets of Stat3 by using an engineered mouse ES cell line treated with GCSF and LIF plus PD0325901 |
|