|
| Status |
Public on Jun 14, 2012 |
| Title |
LIF/PD rep2 |
| Sample type |
RNA |
| |
|
| Source name |
ESC treated LIF/PD for 1hr
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
cell line: ESCs stably stably expressing a chimeric receptor (GRgp-Y118F)
agent: LIF/PD
|
| Treatment protocol |
GCSF (homemade), LIF (homemade), PD0325901 (Stemgent 1 µM)
|
| Growth protocol |
GMEM medium (Sigma) supplemented with 10% fetal bovine serum (FBS) (Hyclone), 2 mM L-glutamine (Gibco), 1 mM sodium pyruvate (Gibco), 1% nonessential amino acids (Gibco), 0.1 mM β-mercaptoethanol (Sigma)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using TRIzol (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
Performed by UCLA DNA Microarray core facility
|
| |
|
| Hybridization protocol |
Performed by UCLA DNA Microarray core facility
|
| Scan protocol |
Performed by UCLA DNA Microarray core facility
|
| Description |
Mouse embryonic stem cell, B6
|
| Data processing |
The data analysis was performed by using Microarray Я US program provided by USC Norris Medical Library
|
| |
|
| Submission date |
Jun 14, 2012 |
| Last update date |
Jun 14, 2012 |
| Contact name |
Qilong Ying |
| Organization name |
USC
|
| Lab |
Qilong Ying
|
| Street address |
1425 san pablo st BCC509b
|
| City |
los angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE38719 |
Identify the downstream targets of Stat3 by using an engineered mouse ES cell line treated with GCSF and LIF plus PD0325901 |
|
