|
| Status |
Public on Sep 05, 2013 |
| Title |
liver_HFD_3A |
| Sample type |
RNA |
| |
|
| Source name |
liver_HFD
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
age: 21 weeks
tissue: liver
pre-treated with: high-fat diet (HFD) for 12 wks
treated with: HFD without compound for 3wks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Liver tissue was first lysed and homogenized in RLT buffer (QIAGEN) with 5 mm steel beads at 20 Hz for 4 min (TissueLyser, QIAGEN). Total RNA was isolated using the RNeasy Mini Kit (QIAGEN) according to their manual. Genomic DNA was digested on column using the DNase-Set (QIAGEN). Quality control was performed with Agilent Bioanalyser
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
replicate 3
|
| Data processing |
The data were normalized using cubic spline normalization implemented in Illumina GenomeStudio V2011.1.
|
| |
|
| Submission date |
Jun 21, 2012 |
| Last update date |
Sep 05, 2013 |
| Contact name |
Christopher Weidner |
| E-mail(s) |
weidner@molgen.mpg.de
|
| Organization name |
Max Planck-Institute for Molecular Genetics
|
| Department |
Otto Warburg Laboratory
|
| Lab |
Sauer
|
| Street address |
Ihnestraße 63
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE38853 |
Protein sets define disease states and predict in vivo effects of drug treatment [Liver A] |
| GSE38856 |
Protein sets define disease states and predict in vivo effects of drug treatment |
|
