|
| Status |
Public on Oct 01, 2013 |
| Title |
clam exposed for 5 days to 1000 µg IBU/l [1000_T3_03] |
| Sample type |
RNA |
| |
|
| Source name |
digestive gland of Manila clam exposed for 5 days to 1000 µg IBU/l
|
| Organism |
Ruditapes philippinarum |
| Characteristics |
time point: 5 days
agent: 1000 µg IBU/l
|
| Treatment protocol |
digestive gland of Manila clam exposed for 5 days to 1000 µg IBU/l Clams were maintained in glass aquaria (without sediment) containing aerated seawater (1 l per animal) in the same thermo-haline conditions that were used during 7 days of acclimatization. Every day, seawater was changed and IBU and microalgae (I. galbana, at an initial concentration of about 150,000 cells/l) were added.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted according to QiAgen RNeasy MiniKit protocol for tissue samples.
|
| Label |
Cyanine 3
|
| Label protocol |
Amplified and labeled according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol.
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol.
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%).
|
| Data processing |
Data processed with FeatureExtraction v9.5 and quantile normalized using R2.6.1 statistical software.
|
| |
|
| Submission date |
Jun 21, 2012 |
| Last update date |
Oct 01, 2013 |
| Contact name |
Massimo Milan |
| E-mail(s) |
massimo.milan@unipd.it
|
| Phone |
+39 0498272941
|
| Organization name |
University of Padova
|
| Department |
Dept. of Department of Public Health, Comparative Pathology,and Veterinary Hygiene
|
| Street address |
Viale dell'Università 16
|
| City |
Legnaro |
| State/province |
PD |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL15723 |
| Series (1) |
| GSE38863 |
Transcriptome analysis of Manila clam (Ruditapes philippinarum) digestive gland after exposure to different concentrations of ibuprofen. |
|
