|
Status |
Public on Mar 07, 2006 |
Title |
HCT116p53- hypoxia 12hrs rep1 |
Sample type |
RNA |
|
|
Source name |
HCT116 p53 null cells exposed to hypoxia 12hrs
|
Organism |
Homo sapiens |
Characteristics |
Cell Line: HCT116 (p53 null) Cell Type: colon carcinoma
|
Treatment protocol |
Cells in monolayer culture were exposed to <0.01% oxygen in an anoxic incubator for the indicated times.
|
Growth protocol |
Standard monolayer tissue culture
|
Extracted molecule |
total RNA |
Extraction protocol |
Affymetrix Genechip Protocol
|
Label |
biotin - streptavidin phycoerythrin (Affymetrix)
|
Label protocol |
Affymetrix Genechip Protocol
|
|
|
Hybridization protocol |
Affymetrix Genechip Protocol
|
Scan protocol |
Affymetrix Genechip Protocol
|
Description |
Affymetrix Genechip Protocol
|
Data processing |
Affymetrix Microarray Suite version 4
|
|
|
Submission date |
Feb 03, 2006 |
Last update date |
Feb 08, 2006 |
Contact name |
Nicholas Denko |
E-mail(s) |
ndenko@stanford.edu
|
Organization name |
Stanford University
|
Department |
Radiation Oncology
|
Street address |
Rm 1245 269 campus dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305 |
Country |
USA |
|
|
Platform ID |
GPL3423 |
Series (1) |
GSE4186 |
Hypoxic changes in gene expression: effect of vhl and p53 genotype |
|