|
| Status |
Public on Dec 31, 2012 |
| Title |
E12L12D_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Hypocrea jecorina CBS999.97 delta-env1
|
| Organism |
Trichoderma reesei |
| Characteristics |
genotype/variation: delta-env1
condition: 12L12D
|
| Treatment protocol |
The Mycelia were freeze-dried and ground in liquid nitrogen using a mortar and pestle.
|
| Growth protocol |
Hypocrea jecorina CBS999.97(MAT1-2) wild-type and Hypocrea jecorina CBS999.97 (MAT1-2) env1 delet strain were inculated in the center of the cellophane-covered malt extract agar (MEA) plate and incubated in a plant growth chamber at 25℃ for 7.25 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using TRI reagent® soln (Ambion), and purified using the RNeasy® plant Mini Kit (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol.(NimbleGen Arrays User’s Guide Gene Expression Arrays ,V6.0)
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol.(NimbleGen Arrays User’s Guide Gene Expression Arrays,V6.0 )
|
| Scan protocol |
The protocol for scanning one-color NimbleGen arrays with a GenePix 4000B Scanner and associated software Genepix6.0. (NimbleGen Arrays User’s Guide: Gene Expression Analysis v3.0)
|
| Data processing |
NimbleScan software (V2.6) was used to obtain raw data(.pair) and converte to gene expression value (.calls). Quantile normalization algorithm was used for normalization methods.
|
| |
|
| Submission date |
Jul 05, 2012 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Ting-Fang Wang |
| E-mail(s) |
tfwang@gate.sinica.edu.tw
|
| Phone |
+8662-2789-9188
|
| Organization name |
Academia Sinica
|
| Department |
Institute of Molecular Biology
|
| Street address |
Academia Sinica. 128 Sec. 2, Academia Rd
|
| City |
Taipei |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL10642 |
| Series (1) |
| GSE39111 |
Expression analysis of Hypocrea jecorina CBS999.97(MAT1-2) wild-type and delta-env1 strains in four different light and darkness conditions |
|
