|
| Status |
Public on Jul 05, 2012 |
| Title |
liver-HCV-Non Tumor-rep5 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
liver-HCV-Non Tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver
tissue subtype: Non Tumor
|
| Growth protocol |
The study was approved by the Institutional Review Board at Washington University in St. Louis and all patients were enrolled after informed consent.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Liver biopsy that were snap frozen obtained from HCV or control normal liver, were used to isolate RNA using Trizol reagent (Invitrogen, Carlsbad, CA) as per manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Samples were labeled according to microRNA expression profiling protocol from Illumina.
|
| |
|
| Hybridization protocol |
Samples were hybridized onto beadchips according to microRNA expression profiling protocol from Illumina.
|
| Scan protocol |
Arrays were scanned on an Illumina BeadArray Reader. Laser power and PMT voltage were kept constant for Cy3 scans. Images were quantitated by Illumina Beadscan, v3. Quantitated data was imported into Beadstudio software. On-slide spot replicates were averaged by Beadstudio and individual spot data was reported
|
| Data processing |
The data were normalized using quantile normalization implemented in Illumina Genome studio version 1.7.0.
|
| |
|
| Submission date |
Jul 05, 2012 |
| Last update date |
Jul 05, 2012 |
| Contact name |
Nobish Varghese |
| E-mail(s) |
nvarghese@path.wustl.edu
|
| Organization name |
Washington University in St.Louis
|
| Street address |
4444 Forest Park Ave.
|
| City |
StLouis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE38226 |
MicroRNA profiling in TGFβ signaling and development of fibrosis. |
|
