|
| Status |
Public on Apr 21, 2014 |
| Title |
13812 TST+ |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral Blood, subject 13812
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: whole blood
disease status: TST+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction of human blood samples were performed using Trizol LS (Invitrogen), according to manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Labelling was perfomed using the miRNA Complete Labeling and Hyb Kit (Agilent Technologies) according supplier's recommendations.
|
| |
|
| Hybridization protocol |
Labeled samples were mixed and hybridized using 1x Hi hybridization buffer together with blocking buffer according to the supplier`s recommendations (Agilent Technologies). Washing of the hybridized arrays was done with the SSC protocol according supplier`s protocol (Agilent Technologies).
|
| Scan protocol |
Scanning of microarrays was performed with 5 µm resolution and extended range using a DNA microarray laser scanner (Agilent Technologies).
|
| Description |
miRNA expression in whole blood
|
| Data processing |
Raw microarray image data were analyzed with the Image Analysis / Feature Extraction software G2567AA (Version A.10.5.1) according supplier's recommendations (Agilent Technologies). Data analysis was carried out on the Rosetta Resolver gene expression data management and analysis system (Build 7.2.2.0.SP1.31) on reporter level.
|
| |
|
| Submission date |
Jul 06, 2012 |
| Last update date |
Apr 21, 2014 |
| Contact name |
Hans-Joachim Mollenkopf |
| E-mail(s) |
mollenkopf@mpiib-berlin.mpg.de
|
| Phone |
+49 30 28460 482
|
| Organization name |
Max-Planck-Institute for Infection Biology
|
| Lab |
Microarray/Genomics Core Facility
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL7731 |
| Series (1) |
| GSE39163 |
MicroRNA miR-223 constrains lethal inflammation during Mycobacterium tuberculosis infection |
|
