|
| Status |
Public on Aug 29, 2018 |
| Title |
HUVEC JAG1 siRNA + IL-1β 24 hours rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Human Umbilical Vein Endothelial Cells (HUVEC)
|
| Organism |
Homo sapiens |
| Characteristics |
sirna: JAG1
il-1β stimulation: +
cell type: pool of 7 HUVEC; confluent at harvest
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with TRI Reagent, followed by clean-up with QIAGEN Rneasy mini kit in accordance with the prescribed protocol provided with the kit. Guality control was performed with the 2100 Bioanalyzer, RIN values were > 9.0 for all samples.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina®TotalPrep™-96 RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The raw data was pre-processed by Illumina’s GenomeStudio software V2011.1, Gene Expression module v. 1.9.0.
|
| |
|
| Submission date |
Jul 09, 2012 |
| Last update date |
Aug 29, 2018 |
| Contact name |
Guttorm Haraldsen |
| E-mail(s) |
guttorm.haraldsen@rr-research.no
|
| Organization name |
Oslo University Hospital
|
| Department |
Department for Pathology
|
| Lab |
LIIPAT
|
| Street address |
Sognsvannsveien 20
|
| City |
OSLO |
| State/province |
OSLO |
| ZIP/Postal code |
0027 |
| Country |
Norway |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE39180 |
Genes regulated/modulated by jagged1 in endothelial cells during inflammation |
| GSE85987 |
Inhibition of endothelial Notch signaling attenuates inflammation |
|
