|
| Status |
Public on Jul 19, 2012 |
| Title |
Nkx3.1CE2/+; Ptenf/f; BrafCA/+ (RAPPD treated)(1) |
| Sample type |
RNA |
| |
|
| Source name |
Anterior Prostate
|
| Organism |
Mus musculus |
| Characteristics |
tissue: anterior prostate
treatment: Rapamycin+PD0325901
sample_id: 7980
|
| Treatment protocol |
For treatment, Rapamycin and/or PD0325901 (or vehicle) were delivered for 5 consecutive days with 2 days off for a period of four weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the MagMAX-96 RNA isolation kit (Ambion). Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Amplification and Labeling was done using the Illumina TotalPrep-96 Amplification kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scaning protocol
|
| Description |
5003139029_D
|
| Data processing |
Data were loaded, normalized, and analyzed with Illumina Expression FileCreator and Illumina Normalizer in GenePattern
|
| |
|
| Submission date |
Jul 09, 2012 |
| Last update date |
Jul 19, 2012 |
| Contact name |
Antonina Mitrofanova |
| E-mail(s) |
mitrofanova.antonina@hotmail.com
|
| Organization name |
Rutgers University
|
| Street address |
65 Bergen Street, Rm 923B
|
| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07107 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE39190 |
B-Raf activation cooperates with PTEN loss to drive c-Myc expression in advanced prostate cancer |
|
