|
| Status |
Public on Sep 30, 2012 |
| Title |
Kas_-Fe_24h_Roots_r1 |
| Sample type |
RNA |
| |
|
| Source name |
Kas roots on minus iron media for 24h
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: Kas
time: 24
iron: Minus
|
| Treatment protocol |
24 d old plants were transferred to treatments of +Fe (50 µM Fe(III)-EDDHA) or -Fe for 24 or 48 h.
|
| Growth protocol |
Seeds of Arabidopsis thaliana ecotypes Kas-1 and Tsu-1 were imbibed in 0.1% agar at 4°C for 72 h, planted onto rockwool, and grown on hydroponic solution. Lighting was provided at a photoperiod of 16 h of 150 µmol m-2 s-1 4,100K fluorescent light. The ecotypes used are Kas-1 and Tsu-1 for our study, the citation of Schuler (2011) used Columbia-0.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from whole rosettes using the RNeasy Plant Kit (Qiagen), and quality was assessed using the Agilent Bioanalyzer 2100.
|
| Label |
biotin
|
| Label protocol |
Done according the manufacturer’s protocol
|
| |
|
| Hybridization protocol |
Done according the manufacturer’s protocol
|
| Scan protocol |
Done according the manufacturer’s protocol
|
| Description |
gene expression data from Kas roots on minus iron media for 24h
|
| Data processing |
R/Bioconductor. Normalized with rma
|
| |
|
| Submission date |
Jul 11, 2012 |
| Last update date |
Sep 30, 2012 |
| Contact name |
Samuel Alonzo McInturf |
| Organization name |
University of Missouri - Columbia
|
| Department |
Plant Sciences
|
| Lab |
Dr. David Mendoza-Cózatl
|
| Street address |
1201 Rollins Street
|
| City |
Columbia |
| State/province |
Missouri |
| ZIP/Postal code |
65201 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE39268 |
Rosette iron deficiency transcript profiling in Arabidopsis thaliana ecotypes Kas-1 and Tsu-1 |
|
