|
| Status |
Public on Dec 31, 2006 |
| Title |
Paerug.exoU3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mouse pooled whole lung lung reference
|
| Organism |
Mus musculus |
| Characteristics |
tissue: whole lung
type: pooled reference RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
cDNA reverse transcribed and indirectly labelled with amino-allyl Cy3 or Cy5
|
| |
|
| Channel 2 |
| Source name |
whole lung
|
| Organism |
Mus musculus |
| Characteristics |
strain: BALB/c
tissue: whole lung
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
cDNA reverse transcribed and indirectly labelled with amino-allyl Cy3 or Cy5
|
| |
|
| |
| Hybridization protocol |
Samples were hybridized for 20h at 63 degrees in a solution containing 2.8 SSC,0.025 M HEPES pH7.0,0.2% SDS,1 μg/μL yeast tRNA,and 0.6 μg/μL human Cot-1 DNA
|
| Scan protocol |
Scanned on an Axon 4000AB scanner.
Images were quantified using GenePix 6.0 software.
|
| Description |
11Mm_183.gpr
P. aeruginosa exoU mutant strain (PA103: 5 x 107 cfu in 50 µl) was instilled intratracheally into BALB/c mice via the oropharynx. Mice were killed 2 h after treatment. Rep 3
|
| Data processing |
LOWESS normalized, no background subtraction was used
|
| |
|
| Submission date |
Feb 10, 2006 |
| Last update date |
Apr 23, 2009 |
| Contact name |
Andrea J Barczak |
| Organization name |
UCSF Sandler Center Functional Genomics Core Facility
|
| Street address |
1550 4th St Rock Hall 545
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL3331 |
| Series (1) |
| GSE4231 |
Comparison of Bleo, Asthma, TH2 and Infection Models |
|
