|
Status |
Public on Apr 15, 2014 |
Title |
Case NE 2 |
Sample type |
RNA |
|
|
Source name |
NE Tissue
|
Organism |
Homo sapiens |
Characteristics |
group: BE case cell type: NE
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA and following clean-up with Qiagen Rneasy Mini Kit were performed according to the manufacturers instructions
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
|
|
Hybridization protocol |
20 ug fragmented cRNA were hybridized for 16 hr at 45C on GeneChip H133-A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip Scanner 3000.
|
Description |
Gene expression data
|
Data processing |
Gene expression data were processed and normalized using Bioconductor Affy package, based on the Robust Multichip Average (RMA) for single-channel Affymetrix chips
|
|
|
Submission date |
Jul 19, 2012 |
Last update date |
Apr 15, 2014 |
Contact name |
Paula Lisa Hyland |
E-mail(s) |
hylandpl@mail.nih.gov
|
Phone |
301-443-8223
|
Fax |
301-402-4489
|
Organization name |
NCI, NIH
|
Department |
DCEG
|
Lab |
GEB
|
Street address |
6120 Executive Blvd
|
City |
Rockville |
State/province |
MD |
ZIP/Postal code |
20895 |
Country |
USA |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE39491 |
GLOBAL CHANGES IN GENE EXPRESSION OF BARRETT'S ESOPHAGUS COMPARED TO NORMAL SQUAMOUS ESOPHAGUS AND GASTRIC CARDIA TISSUES |
|