|
| Status |
Public on Apr 15, 2014 |
| Title |
Case NE 16 |
| Sample type |
RNA |
| |
|
| Source name |
NE Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
group: BE case
cell type: NE
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and following clean-up with Qiagen Rneasy Mini Kit were performed according to the manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
20 ug fragmented cRNA were hybridized for 16 hr at 45C on GeneChip H133-A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip Scanner 3000.
|
| Description |
Gene expression data
|
| Data processing |
Gene expression data were processed and normalized using Bioconductor Affy package, based on the Robust Multichip Average (RMA) for single-channel Affymetrix chips
|
| |
|
| Submission date |
Jul 19, 2012 |
| Last update date |
Apr 15, 2014 |
| Contact name |
Paula Lisa Hyland |
| E-mail(s) |
hylandpl@mail.nih.gov
|
| Phone |
301-443-8223
|
| Fax |
301-402-4489
|
| Organization name |
NCI, NIH
|
| Department |
DCEG
|
| Lab |
GEB
|
| Street address |
6120 Executive Blvd
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20895 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE39491 |
GLOBAL CHANGES IN GENE EXPRESSION OF BARRETT'S ESOPHAGUS COMPARED TO NORMAL SQUAMOUS ESOPHAGUS AND GASTRIC CARDIA TISSUES |
|
