|
| Status |
Public on Apr 05, 2013 |
| Title |
blastomere 1 from embryo 1 E1.C1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
blastomere 1 from embryo 1
|
| Organism |
Homo sapiens |
| Characteristics |
age: day 4 embryo
cell type: blastomere
sample type: blastomere from frozen-thawed day-4 embryo
|
| Treatment protocol |
All blastomeres from nine top-quality day-4 embryos frozen at day 3 of development and four fresh top-quality day-4 embryos that had one-cell biopsy on day 3 for preimplantation genetic diagnosis (PGD) were collected and arrayed.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Blastomeres were collected and the genomic DNA was amplified using the SurePlex DNA Amplification System (BlueGnome, Cambridge, UK), according to the manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Array-CGH was carried out using 24Sure Cytochip microarrays following the standard protocol (BlueGnome 24Sure Protocol, www.cytochip.com).
|
| |
|
| Channel 2 |
| Source name |
Control genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
|
| Treatment protocol |
All blastomeres from nine top-quality day-4 embryos frozen at day 3 of development and four fresh top-quality day-4 embryos that had one-cell biopsy on day 3 for preimplantation genetic diagnosis (PGD) were collected and arrayed.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Blastomeres were collected and the genomic DNA was amplified using the SurePlex DNA Amplification System (BlueGnome, Cambridge, UK), according to the manufacturer’s instructions.
|
| Label |
Cy5
|
| Label protocol |
Array-CGH was carried out using 24Sure Cytochip microarrays following the standard protocol (BlueGnome 24Sure Protocol, www.cytochip.com).
|
| |
|
| |
| Hybridization protocol |
Array-CGH was carried out using 24Sure Cytochip microarrays following the standard protocol (BlueGnome 24Sure Protocol, www.cytochip.com).
|
| Scan protocol |
The slides were scanned using an Agilent dual laser DNA microarray scanner G2566AA (Agilent technologies, Palo Alto, CA, USA).
|
| Description |
frozen-thawed day-4 embryo
|
| Data processing |
The scan images were analysed using BlueFuse Software (BlueGnome).
|
| |
|
| Submission date |
Jul 19, 2012 |
| Last update date |
Apr 05, 2013 |
| Contact name |
Claudia Spits |
| E-mail(s) |
claudia.spits@vub.be
|
| Organization name |
Vrije Universiteit Brussel
|
| Department |
Research Group of Reproduction and Genetics
|
| Lab |
Research Group of Reproduction and Genetics
|
| Street address |
Laarbeeklaan 103
|
| City |
Brussels |
| ZIP/Postal code |
1090 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL15832 |
| Series (1) |
| GSE39518 |
array-CGH on single blastomeres from day-4 human preimplantation fresh and frozen embryos |
|
