|
| Status |
Public on Jul 21, 2012 |
| Title |
HL1 Day4 Rep2 |
| Sample type |
SRA |
| |
|
| Source name |
HL1 Cardiomyocyte cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: HL-1
time: 4 days in culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from HL-1 cultures using the TRIzol® reagent. Small RNA <70 nt was enriched by flashPAGE™ electrophoresis. Libraries were created with the SOLiD™ Small RNA Expression Kit and sequenced using SOLiD™ version 2 reagents.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
AB SOLiD System 3.0 |
| |
|
| Data processing |
The SOLiD™ Small RNA Pipeline was used to map tags to miRBase version 16 and the mouse genome (mm37 assembly).
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include raw tag count for each sequence observed
|
| |
|
| Submission date |
Jul 20, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Hardip Rameshbhai Patel |
| E-mail(s) |
hardip.patel@anu.edu.au
|
| Organization name |
The Australian National University
|
| Department |
Genome Biology Department
|
| Lab |
Genome Discovery Unit & RNA Biology Laboratory
|
| Street address |
Bld 131, ANU Campus,
|
| City |
Acton |
| State/province |
Australian Capital Territory |
| ZIP/Postal code |
0200 |
| Country |
Australia |
| |
|
| Platform ID |
GPL9318 |
| Series (1) |
| GSE39531 |
Complexity of Murine Cardiomyocyte miRNA Biogenesis, Sequence Variant Expression and Function |
|
| Relations |
| BioSample |
SAMN02197499 |
| SRA |
SRX036575 |
