|
| Status |
Public on Jul 25, 2012 |
| Title |
71XM_UNINTERRUPTED |
| Sample type |
RNA |
| |
|
| Source name |
vastus lateralis muscle
|
| Organism |
Homo sapiens |
| Characteristics |
individual: 71XM
gender: MALE
age: 49
weight (kg): 124.8
bmi: 33.9
treatment protocol: uninterrupted sitting
tissue: vastus lateralis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen muscle with TRIzol reagent (InVitrogen, Carlsbad, CA, USA) using the manufacturer’s recommended protocol. RNA quality was determined by the MultiNA system (Shimadzu Biotech, Sydney, NSW, Australia).
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was produced by means of a linear amplification kit (AMIL1791; Ambion, Austin, TX, USA) using 250ng of quality checked total RNA as input.
|
| |
|
| Hybridization protocol |
Hybridization to the Illumina Human HT-12 v4 BeadChip was for 16 hours at 58ºC on a BeadChip Hyb Wheel using 1,500ng biotin-labeled cRNA as input. The chip was then washed, blocked and stained with Cy3-streptavidin (Amersham Bioscience, Piscataway, NJ, USA) according to manufacturer’s protocols (Illumina, SanDiego, CA, USA).
|
| Scan protocol |
The iScan Control Software V1.6.20.7 (Illumina) together with the Illumina iSCAN platform (Illumina) was used for RNA quantification.
|
| Description |
uninterrupted sitting_ repl3
SAMPLE 8
|
| Data processing |
Processing and analysis of the microarray data were performed with the Illumina Beadstudio 3.1.3.0 software. Raw data were background subtracted and normalized using the “Quantile” algorithm.
|
| |
|
| Submission date |
Jul 23, 2012 |
| Last update date |
Jul 25, 2012 |
| Contact name |
Celine Latouche |
| E-mail(s) |
celine.latouche@bakeridi.edu.au
|
| Phone |
0385321375
|
| Organization name |
Baker IDI Heart and Diabetes Institute
|
| Lab |
Metabolic and Vascular Physiology
|
| Street address |
75 Commercial Road
|
| City |
Melbourne |
| State/province |
VICTORIA |
| ZIP/Postal code |
3181 |
| Country |
Australia |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE39576 |
Effects of breaking up prolonged sitting on skeletal muscle gene expression |
|
