|
| Status |
Public on Jul 27, 2012 |
| Title |
TaxP |
| Sample type |
RNA |
| |
|
| Source name |
Stably transfected Jurkat_pCMV-Tax
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Jurkat (CD4 T cell)
treatment: stably integrated with pCMV-Tax
|
| Treatment protocol |
Jurkat cells were transfected with pCMV-Bam-Tax plasmids, and selected by G418. cells stably expresse Tax were selected and maintained in the presence of G418.
|
| Growth protocol |
These cells were maintained in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum, glutamine, G418 and antibiotics at 37 °C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the TaxP cell was isolated using the Trizol (Invitrogen life technologies) according to the supplier's protocols.
|
| Label |
Cy3
|
| Label protocol |
standard Phalanx protocol (See http://www.phalanxbiotech.com/)
|
| |
|
| Hybridization protocol |
standard Phalanx protocol (See http://www.phalanxbiotech.com/)
|
| Scan protocol |
standard Phalanx protocol (See http://www.phalanxbiotech.com/)
|
| Data processing |
The data were analyzed by GenePix Pro 6.0
|
| |
|
| Submission date |
Jul 24, 2012 |
| Last update date |
Jul 27, 2012 |
| Contact name |
Hui Wang |
| E-mail(s) |
wanghui@xxmu.edu.cn
|
| Phone |
86 373 3029203
|
| Fax |
86 373 3029203
|
| Organization name |
Xinxiang Medical University
|
| Department |
Research Center for Immunology
|
| Street address |
eastern section of Jinsui road
|
| City |
Xinxiang |
| State/province |
Henan Province |
| ZIP/Postal code |
453003 |
| Country |
China |
| |
|
| Platform ID |
GPL6254 |
| Series (1) |
| GSE39601 |
Profile of gene expression in Tax positive (TaxP) cells compared with Tax negative (TaxN) cells. |
|
