|
Status |
Public on Feb 05, 2013 |
Title |
DHT rep 2 |
Sample type |
RNA |
|
|
Source name |
LNCaP-1F5 cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: LNCaP-1F5 treatment: DHT
|
Treatment protocol |
LNCaP-1F5 cells were grown in charcoal stripped medium for 3 days followed by treated with vehicle or 100 nM DHT or 100 nM Dex or 1000 nM CPA or 1000 nM (RU486) or combination of 100 nM DHT+Dex for 24 hours followed by RNA isolation.
|
Growth protocol |
LNCaP-1F5 cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS), 2 mM L-glutamine, and 10 mM HEPES supplemented with antibiotics (penicillin and streptomycin).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kitwith DNaseI treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 2 treated with DHT for 24 hours 5298416049_E
|
Data processing |
The data were normalised using quantile normalisation with BeadStudio Program from Illumina.
|
|
|
Submission date |
Jul 25, 2012 |
Last update date |
Feb 05, 2013 |
Contact name |
Olli A. Jänne |
E-mail(s) |
olli.janne@helsinki.fi
|
Phone |
+358919125040
|
Organization name |
University of Helsinki
|
Department |
Inst. of Biomedicine/Physiology
|
Lab |
Androgen Receptor Laboratory
|
Street address |
Haartmaninkatu-8
|
City |
Helsinki |
ZIP/Postal code |
FI-00014 |
Country |
Finland |
|
|
Platform ID |
GPL6947 |
Series (2) |
GSE39654 |
FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells (mRNA) |
GSE39880 |
FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells |
|