|
| Status |
Public on Oct 01, 2015 |
| Title |
Whole leaves compatible interaction 48h |
| Sample type |
RNA |
| |
|
| Source name |
Entire detached leaves inoculated for 48h with M. larici-populina isolate 98AG31
|
| Organism |
Populus trichocarpa x Populus deltoides |
| Characteristics |
cultivar: 'Beaupré'
tissue: entire detached leaves
treatment: inoculation with Melampsora larici-populina isolate 98AG31
treatment duration: 48h
|
| Biomaterial provider |
Institut National de la Recherche Agronomique (INRA Nancy, France)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction and quantity/quality control were performed as described in Rinaldi et al. 2007. RNA quality was verified by Experion StdSens capillary gels (Bio-Rad). Double-stranded (ds) cDNA were synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech, Saint-Germain-en-Laye, France), following manufacturer's instructions, with 0.5 μg of starting total RNA and 19 cycles of PCR amplification in 25μL. PCR products were purified with the QIAquick PCR Purification Kit (Qiagen, Couteboeuf, France) and quantified with a NanoDrop 1000 spectrophotometer (Labtech, Palaiseau, France).
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimbleGen Company
|
| |
|
| Hybridization protocol |
Hybridization by NimbleGen Company
|
| Scan protocol |
Scanning by NimbleGen Company
|
| Data processing |
Expression values reflect across-array quantile normalization and gene calls generated using the Robust Multichip Average (RMA). For information on RMA, see Biostatistics 2003 Apr; 4(2): 249-264.
|
| |
|
| Submission date |
Jul 30, 2012 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
annegret.kohler@inrae.fr
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
| |
|
| Platform ID |
GPL13485 |
| Series (1) |
| GSE39727 |
Analysis of wound-induced transcripts in poplar leaf disks versus whole leaves |
|
