|
| Status |
Public on Sep 10, 2012 |
| Title |
SPORE MM_1509_3 |
| Sample type |
RNA |
| |
|
| Source name |
CD138 purified myeloma plasma cells
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: newly diagnosed multiple myeloma
|
| Treatment protocol |
All patient samples were from newly diagnosed patients with multiple myeloma. Samples were collected before initiation of primary treatment and CD138 purified plasma cells were isolated. All patients received triple drug regime - Vincristine, Adriamycin, and Dexamethasone (VAD) - as induction therapy followed by Autologous Stem Cell Transplant (ASCT) as a maintenenace therapy. 'Treatment response' was measured post-ASCT.
Treatment responses: Complete Response (CR); Very Good Partial Response (VGPR); Partial Response (PR); No Response, Stable disease (NR); No Response, Progressive disease (Prog).
|
| Growth protocol |
not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix), and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were analyzed using the Affymetrix GeneChip Human Exon 1.0 ST Array (Affymetirx, Santa Clara, CA) according to the manufacturer's instructions.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instructions (Affymetrix).
|
| Data processing |
The aroma.affymetrix package was utilized for pre-processing and RMA normalization of the data.
HuEx-1_0-st-v2,core,A20071112,EP.cdf
|
| |
|
| Submission date |
Jul 30, 2012 |
| Last update date |
Mar 14, 2014 |
| Contact name |
Nikhil C Munshi |
| E-mail(s) |
nikhil_munshi@dfci.harvard.edu
|
| Phone |
617-632-4218
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Hematologic Oncology
|
| Street address |
450 Brookline Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE39754 |
Gene Expression profiling of Multiple Myeloma |
|
