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Sample GSM978655 Query DataSets for GSM978655
Status Public on Sep 10, 2012
Title SPORE MM_2789_3
Sample type RNA
 
Source name CD138 purified myeloma plasma cells
Organism Homo sapiens
Characteristics diagnosis: newly diagnosed multiple myeloma
treatment_response: CR
Treatment protocol All patient samples were from newly diagnosed patients with multiple myeloma. Samples were collected before initiation of primary treatment and CD138 purified plasma cells were isolated. All patients received triple drug regime - Vincristine, Adriamycin, and Dexamethasone (VAD) - as induction therapy followed by Autologous Stem Cell Transplant (ASCT) as a maintenenace therapy. 'Treatment response' was measured post-ASCT.
Treatment responses: Complete Response (CR); Very Good Partial Response (VGPR); Partial Response (PR); No Response, Stable disease (NR); No Response, Progressive disease (Prog).
Growth protocol not applicable
Extracted molecule total RNA
Extraction protocol Total RNA extracted
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix), and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were analyzed using the Affymetrix GeneChip Human Exon 1.0 ST Array (Affymetirx, Santa Clara, CA) according to the manufacturer's instructions.
Scan protocol Array scanning was performed according to the manufacturer's instructions (Affymetrix).
Description ifm_100
Data processing The aroma.affymetrix package was utilized for pre-processing and RMA normalization of the data.
HuEx-1_0-st-v2,core,A20071112,EP.cdf
 
Submission date Jul 30, 2012
Last update date Mar 14, 2014
Contact name Nikhil C Munshi
E-mail(s) nikhil_munshi@dfci.harvard.edu
Phone 617-632-4218
Organization name Dana-Farber Cancer Institute
Department Hematologic Oncology
Street address 450 Brookline Avenue
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL5175
Series (1)
GSE39754 Gene Expression profiling of Multiple Myeloma

Data table header descriptions
ID_REF
VALUE rma normalized intensity value

Data table
ID_REF VALUE
2315251 11.26557255
2315373 53.78388977
2315554 149.3987122
2315633 144.6112366
2315674 127.1514511
2315739 171.4537659
2315894 216.4364624
2315918 79.07847595
2315951 281.749054
2316069 171.6411285
2316218 69.38721466
2316245 178.2582092
2316379 413.5801697
2316558 398.2561646
2316605 120.5645523
2316746 286.5262146
2316905 59.75279617
2316953 93.9575882
2317246 94.57062531
2317317 111.0923538

Total number of rows: 18708

Table truncated, full table size 363 Kbytes.




Supplementary file Size Download File type/resource
GSM978655_SPORE_MM_2789_3.CEL.gz 23.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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