NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM98068 Query DataSets for GSM98068
Status Public on Dec 30, 2007
Title C3H_Lung_NoIrradiation
Sample type RNA
 
Channel 1
Source name Lung_NoIrradiation
Organism Mus musculus
Characteristics 'Strain: C3H/HeMs', 'Gender: female', 'Age: 12~13 week'
Treatment protocol No treatment
Extracted molecule total RNA
Extraction protocol ISOGEN(Wako pure chemical)+RNeasy(QIAGEN)
Label Cy3
Label protocol Agilent Fluorescent Direct Label Kit Protocol (June 2003 Rev 2.1), Publication Number: G2555-98003_JUNE_2003
 
Channel 2
Source name control RNA mixture
Organism Mus musculus
Characteristics Ten of First Choice total RNA (Ambion, Inc.) derived from ten organs(midterm embryo, thymus, ovary, testicle, spleen, lung, heart, liver, kidney, brain) were equally mixed.
Biomaterial provider Ambion, Inc.
Extracted molecule total RNA
Label Cy5
Label protocol Agilent Fluorescent Direct Label Kit Protocol (June 2003 Rev 2.1), Publication Number: G2555-98003_JUNE_2003
 
 
Hybridization protocol cDNA Microarray Kit Protocol (Double barcoded slide) Version 7.0, Publication Number: G4100-98000
Scan protocol Agilent Microarray Scanner User Manual (6.3, 5th edition, December 2003), Publication Number: G2566-90009 10µm resolution, 100% PMT
Description In channel 1, total RNA was purified and pooled from 3 mice
Data processing Feature Extraction Software (Agilent Technologies)
 
Submission date Feb 23, 2006
Last update date Apr 22, 2007
Contact name Shuhei Noda
E-mail(s) s_noda@nirs.go.jp
Organization name National institute of radiological sciences
Department Frontier research center
Street address Anagawa 4-9-1
City Chiba
State/province Chiba
ZIP/Postal code 2658333
Country Japan
 
Platform ID GPL872
Series (1)
GSE4312 Radiation-induced genes commonly expressed in lung and skin among three strains of mice.

Data table header descriptions
ID_REF
VALUE LogRatio. log10(Dye normalized red(Cy5) Signal/Dye normalized green(Cy3) Signal) per feature
gMeanSignal Raw mean signal of feature in green(Cy3) channel
rMeanSignal Raw mean signal of feature in red(Cy5) channel
gBGMeanSignal Mean local background signal (local to corresponding feature) in green(Cy3) channel
rBGMeanSignal Mean local background signal (local to corresponding feature) in red(Cy5) channel

Data table
ID_REF VALUE gMeanSignal rMeanSignal gBGMeanSignal rBGMeanSignal
1 0.0454022 62868.4 52732.4 628.355 428.248
2 -0.338639 866.038 580.846 612.331 419.787
3 -0.240695 756.667 565.875 615.3 421.443
4 -0.281883 871.92 617.84 612.518 425.759
5 -0.359461 662.87 461.043 616.756 439.526
6 -0.21472 824.923 630 633.565 461.217
7 -0.294652 656.593 476.148 630.319 470.474
8 0.639641 1293.36 4717 610.481 450.615
9 -0.0164089 850.458 826.042 616.82 437.898
12 -0.205344 4640.21 2994.17 628.226 467.752
14 0.273194 5356.75 9534.29 628.028 453.567
15 -0.103025 5334.83 4191.96 646.359 468.519
16 0.23747 22230.4 32298.3 653.849 472.349
17 0.0931914 11716.4 13088.3 674.857 473.414
19 0.655798 8820.04 36068.1 768.313 524.731
20 1.16553 968.091 9713.18 728.099 523.527
21 0.626615 8689.22 33280.4 691 504.771
22 0.0601593 2133 2498.17 676.746 484.556
23 1.76441 988.16 38892.6 673.5 483.023
24 0.499813 24786.6 64876.2 664.315 483.408

Total number of rows: 9275

Table truncated, full table size 416 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.