|
| Status |
Public on Jan 10, 2013 |
| Title |
PMN Sm-Mab, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
neutrophils, smooth Mab
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: blood neutrophils
disease status: healthy
treatment: smooth M. abscessus
treatment: 2 hrs
|
| Treatment protocol |
Bacteria (or saline) were incubated at 10 bacteria per neutrophil for 2 hours.
|
| Growth protocol |
Freshly isolated human neutrophils from healthy donors were incubated in RPMI containing 2% heat-inactivated platelet-poor plasma.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol Reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Microarray sample labeling was carried out using standard methods for reverse transcription and one round of in vitro transcription.
|
| |
|
| Hybridization protocol |
Affymetrix HG-U133 Plus 2.0 microarrays were hybridized with 10µg cRNA.
|
| Scan protocol |
GeneChip Scanner 3000 7G (Affymetrix)
|
| Description |
Sm2
Gene expression data from neutrophils after 2h Sm-Mab.
|
| Data processing |
The data were analyzed with Command Console Microarray Suite version 5.0 (MAS 5.0)(Affymetrix).
|
| |
|
| Submission date |
Aug 05, 2012 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Ken Malcolm |
| E-mail(s) |
malcolmk@njhealth.org
|
| Organization name |
National Jewish Health
|
| Street address |
1400 Jackson St
|
| City |
Denver |
| ZIP/Postal code |
80206 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE39889 |
Neutrophil gene expression in response to Mycobacterium abscessus |
|
| Relations |
| Reanalyzed by |
GSE86362 |
