|
| Status |
Public on Jul 06, 2017 |
| Title |
PSB-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Fully expanded leaf from 8 week old plant
|
| Organism |
Brassica oleracea |
| Characteristics |
variety: Purple sprouting Broccoli
age: 8 weeks
tissue: fully expanded leaf
|
| Growth protocol |
Plants were gerown in 17 cm pots containing a peat based compost (Lentse potgrond)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy kit as per manufactures instructions
|
| Label |
biotin
|
| Label protocol |
RNA was amplified, fragmented and labeled using the Ambion WT expression kit.
|
| |
|
| Hybridization protocol |
Samples were hybridised at the NASC microarray core facility according to manufactures' instructions. GeneChips were washed and stained in the Affymetrix Fluidics station 450.
|
| Scan protocol |
Scanning was performed accordinf to manufactures' instruction using an Affymetrix 3000 7G scanner
|
| Description |
P.S.Broccoli-rep2
|
| Data processing |
RNA data was analysed using Genespring GX 11 software (Agilent technologies) using the RMA probe sumarisation algorithm. Each gene signal was then normalised to the median of the gene signal across all samples
|
| |
|
| Submission date |
Aug 07, 2012 |
| Last update date |
Jul 06, 2017 |
| Contact name |
Neil Graham |
| E-mail(s) |
neil.graham@nottingham.ac.uk
|
| Phone |
01159516373
|
| Organization name |
University of Nottingham
|
| Department |
Plant and Crop Sciences
|
| Lab |
Broadley
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| State/province |
Leicestershire |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10733 |
| Series (1) |
| GSE39951 |
Biofumigation effects of wild Brassica oleracea on beneficial soil invertebrates |
|
